Typical parameters for Hyaluronic Acid (Free Acid Form, 16,000 Da batch RX20251029-2).
| Test Item | Result / Specification |
|---|---|
| Physical & Chemical | |
| Appearance | White or almost white powder or granule |
| Glucuronic Acid Content | ≥ 44.5% |
| Hyaluronic Acid Content | ≥ 92.0% |
| Average Molecular Weight | Measured value |
| pH (5 mg/mL, 25°C) | 3.0 – 5.0 |
| Transmittance (5 mg/mL, 25°C) | ≥ 99.0% |
| Loss on Drying | ≤ 10.0% |
| Ash Content | ≤ 5.0% |
| Protein | ≤ 0.1% |
| Heavy Metals | ≤ 20 ppm |
| Microbiological | |
| Total Aerobic Count | ≤ 100 CFU/g |
| Molds & Yeasts | ≤ 50 CFU/g |
| Staphylococcus aureus | Negative/g |
| Pseudomonas aeruginosa | Negative/g |
| Thermotolerant Coliform Bacteria | Negative/g |
| Packaging & Storage | |
| Shelf Life | 36 months |
| Storage | Sealed, cool and dry |
Contains no sodium salt — ash content ≤ 5% (far below the 13–20% of sodium salt forms), suitable for formulations with strict sodium ion limitations
The free acid form has a naturally acidic pH profile, well-suited for formulations maintained in the mildly acidic range — closer to the natural skin pH
High-assay free acid form suitable for research applications and premium formulations requiring precise HA active dose control
Standard grade at 16,000 Da (hydrolyzed); custom grades available from as low as 800 Da (nano) to higher MW ranges — covering different skin penetration depth requirements
Uses the INCI name Hyaluronic Acid rather than Sodium Hyaluronate, meeting specific INCI labeling requirements in certain markets and brand standards
Transmittance ≥ 99%, clear solutions compatible with most aqueous formulation systems — suitable for serums, creams, masks, and research applications
Formulations requiring a mildly acidic environment — such as AHA/BHA or vitamin C serums — where free acid HA avoids the alkaline interference introduced by sodium salt forms
Laboratory and in-vitro research applications requiring precise HA concentrations and defined molecular weights — the free acid form enables accurate preparation of defined-concentration solutions
Grades at 16,000 Da and below exhibit excellent stratum corneum penetration — a key active ingredient in premium anti-aging serums and ampoule formulations
Consumers in certain markets such as the North American natural beauty segment prefer the Hyaluronic Acid INCI label — the free acid form meets this labeling requirement
Request samples of our 16,000 Da free acid HA or discuss custom molecular weight requirements with our team.